Craig Markin is a Fellow in the Manchester Institute of Biotechnology and the Division of Molecular and Cellular Function at the University of Manchester. His group's research is focused on understanding the molecular and physical basis of protein and enzyme function with the goal of addressing the two challenges of making enzyme design and engineering more rational and understanding the biological consequences of disease variants. In his postdoctoral work with Profs. Dan Herschlag and Polly Fordyce at Stanford University, Craig led the development of HT-MEK (High-Throughput Microfluidic Enzyme Kinetics), a novel microfluidics-based assay to express, purify, and quantitatively measure a variety of kinetic and thermodynamic constants for >1000 enzyme variants in parallel. In his graduate work with Prof. Leo Spyracopoulos at the University of Alberta, Craig studied the molecular mechanisms of polyubiquitin chain synthesis and their subsequent recognition by partner proteins in the DNA damage response with the aim of understanding how the kinetics and thermodynamics of these processes dictate biological outcomes.