MAVE resources
Multiplexed assays of variant effects (MAVEs) allow us to assess (in a single experiment) thousands of variants simultaneously. This technique, which harnesses the power of next-gen sequencing, yields large-scale data sets that can shed light on the functional consequences of protein variants and ultimately on the the landscape of human genetic variation.
Note: We are currently working on providing a comprehensive MAVE knowledge base. We hope these preliminary resources will be a helpful starting place!
If you are interested in helping to further develop this resource, please contact project manager Lara Muffley (muffley [@] uw.edu)
MaveDB
A public repository for variant effect maps
pacybara and tileseq
Pacbio barcode clustering and barseq pipeline and TileSeq analysis pipeline for MAVEs (https://github.com/rothlab)
MAVE impute
Impute missing data for variant effect maps
MAVE registry
A collaborative resource for sharing progress on MAVEs
So you want to make a variant effect map…
What tools do I need? What variables should I consider? How do I get started?
Educational Resources
Comprehensive MAVE-relevant reviews Thinking about incorporating MAVEs into your work? Visit our MAVE educational resources page to find comprehensive MAVE-relevant reviews.
Technology and methods development manuscripts Designing a MAVE?
>>Follow this link<< to view granular reviews and seminal papers relevant to methods at each stage of the MAVE development process.
Experimental Resources and Protocols
New! MAVE protocols collection in protocols.io.
https://www.protocols.io/workspaces/atlas-of-variant-effects-alliance
To add your protocol to the collection, please visit this how-to guide.
Educational Videos
Visit (and subscribe to!) our youtube channel (which we jointly manage with CMAP/CEGS)
Learn about MaveDB - the public repository for datasets from Multiplexed Assays of Variant Effect (MAVEs), in this video> “Understanding the functional consequences of every mutation in Your Favourite Gene”
Computational Resources
Variant Effect Predictors https://www.varianteffect.org/veps
MAVE Analysis Tools https://www.varianteffect.org/analysis-tools
Discussion Forums
Atlas of Variant Effects Alliance community slack channel (https://www.varianteffect.org/membership)